Secondary metabolite production by using callus and suspension culture

Steps involved in the production of secondary metabolites:-

1. Selection of desired mother plant
2. Establishment of explant culture
3. Establishment of callus culture
4. Establishment of suspension culture
5. Optimization and scale-up
6. Industrial production

Significance:-

1. Production of flavors
2. Production of fragrances
3. Production of essential oil
4. Production of pigments
5. Production of antimicrobial agents
6. Production of pharmaceutical active ingredients